Coronavirus refers to the ubiquitous single-stranded RNA positive virus. There are already six types of coronaviruses that have been proven to attack humans: OC43, NL63, 229E, and HIU1, as well as two other types of pathogenic coronaviruses that have been discovered in recent years, namely: H. Respiratory
Coronavirus Syndrome (MERS COV) 2012. The new coronavirus (SARS-CoV-2) which broke out in 2019 is the seventh. New coronavirus pneumonia (COVID-19) is a new lung inflammation caused by the coronavirus that people are usually prone to experience. Respiratory droplets and contact are the main routes of transmission. There are some companies that provide ELISA testing service to detect viruses.
Infected patients usually develop symptoms of pneumonia (fever, cough, shortness of breath, etc.), diarrhoea and other gastrointestinal symptoms, followed by severe acute respiratory infections. Some cases experience acute shortness of breath with serious respiratory complications that can even lead to death.
From the COVID-19 outbreak to February 22, 2020, World Health Organization (WHO) statistics show that 77,794 patients have been confirmed as infected, with 76,392 confirmed cases in China. In order to diagnose and accurately record the spread of this virus, doctors need fast and reliable in vivo and in vitro diagnostic methods.
Review of virus detection technology in the laboratory
All substances except prions contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). SARS-CoV-2 contains the only RNA whose specific RNA sequence is a marker to differentiate it from other pathogens.
The most common method used to detect specific sequences of the novel coronavirus is the fluorescent quantitative polymerase chain reaction (PCR). According to the etiologic diagnosis or clinical diagnosis with RT-PCR defects in airway samples or fluid samples, positive results were shown, or the sequence results showed great similarity to SARS-CoV-2, one of which was confirmed cases.